Bio Abstract

26. Anthony T.W. Cheung, Lawrence J.T. Young, Peter C.Y. Chen, Chien Y. Chao, Assane Ndoye, Peter A. Barry, William J. Muller and Robert D. Cardiff. Microcirculation And Metastasis In A New Mouse Mammary Tumor Model System. Int. J. Oncology. 1997. 11:66-77, (ABSTRACT)(ILLUSTRATIONS)

Abstract
Two new metastatic mouse mammary tumor transplant lines have been established in nude mice. The Met-1 line, with the Polyomavirus Middle T (PyV-MT) transgene, metastasized with 100% efficiency. The Db-7 line, expressing a PyV-MT transgene mutated at positions 315 and 322, metastasized with 8.8% efficiency. Histology and computer-assisted intravital microscopy demonstrated that internal microcirculation in Met-1 was more complex than Db-7; Met-1 exhibited higher microvessel density and tortuosity (P<0.0001) . These indices of microvascular complexity correlated with the higher Met-1 metastatic rate (P<0.0001). These two transplantable lines will be useful for investigating the complex relationship between angiogenesis and metastasis.