VISUALIZATION OF THE MICROCIRCULATION

• THE MICE WERE INJECTED WITH FITC.
• THE DYE WAS ALLOWED TO LEAK OUT OF THE VESSEL AND VISUALIZED USING INDIRECT EPIFLOURESCENCE.
• THIS RESULTED IN A REVERSE IMAGE THAT SHOWED THE VESSELS AS DARK LINES AGAINST A LIGHT BACKGROUND.
• THE DIGITAL IMAGES WERE THEN ANALYZED WITH THE VASCAN SOFTWARE PROGRAM
• THE REVERSE EPIFLOUSCENCE MICROSCOPY PERMITTED VISUALIZATION OF THE INTERNAL MICROCIRCULATION UP TO DEPTHS OF 100 MICRON.