ANGIOGENESIS

This page summarizes Dr. Jensen's study of angiogenesis produced by explants of hyperplasias into the anterior chamber of the eye. It was the first to experimentally document angiogenesis in human preneoplasia.

Taken from:

Jensen HM, Chen I, DeVault MR, Lewis AE . Angiogenesis induced by "normal" human breast tissue: a probable marker for precancer. Science 1982 Oct 15;218(4569):293-5
Normal human breast lobules, freshly isolated by precision microdissection of tissue stained with methylene blue chloride, were assayed for their ability to induce neovascularization (angiogenesis) in rabbit irises. Histologically, normal lobules from cancerous breast induced angiogenesis twice as often as lobules from noncancerous breasts, suggesting that preneoplastic transformation is diffuse.

See also:

Brem SS, Jensen HM, Gullino PM Angiogenesis as a marker of preneoplastic lesions of the human breast. Cancer 1978 Jan;41(1):239-44

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Slide 139.
Normal lobules persist in cancer associated breasts.
This phenomenon may be due to their angiogenicity.

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Slide140. Arrows point to two lobules stained supravitally with methylene blue chloride. Photographed at 2x magnification
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Slide 141. 4 such lobules protrude from their surrounding dense stroma Photographed at 6.3 magnification
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Slide 142. A single lobule has been dissected out. Note the delicate network of lymphatics on the surface. Photographed at 6.3x magnification
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Slide 143. Fragments were placed on the iris of the rabbit eye.

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Slide 144. 3 transplants are present on the eye. Photographed at 2x magnification
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Slide 145. The transplant at 10 o'clock is not angiogenic. The one at 2 o'clock has evoked a delicate capillary response on the 3rd day.
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Slide 146. This hyperplastic alveolar nodule from a mouse was highly angiogenic.
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Slide 147. This histologic slide of a "normal" angiogenic lobule shows the transplant sitting on top of the rabbit iris. Photographed at 6.3x magnification

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Slide 148. This histologic slide of a fragment of mild ductal hyperplasia is sitting on top of the rabbit iris. It was angiogenic. Photographed at 6.3x magnification ~ 30% of normal appearing lobules from cancer associated breasts were angiogenic in the rabbit iris assay but only 7% were angiogenic when removed from breast biopsies with no cancer. ~30% of ductal hyperplasia of the mild form were angiogenic.
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Slide 149. This diagram formulates the hypothesis that the angiogenic factor produced by the lobules stimulates fibroblasts to produce excess collagen as seen in fibrocystic change. ~35% of breasts with fibrocystic change have hyperplasias of ductal type.

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slide140.jpg 430.95 Kb Slide140. Arrows point to two lobules stained supravitally with methylene blue chloride. Photographed at 2x magnification	slide141.jpg 272.18 Kb Slide 141. 4 such lobules protrude from their surrounding dense stroma Photographed at 6.3 magnification	slide142.jpg 411.44 Kb Slide 142. A single lobule has been dissected out. Note the delicate network of lymphatics on the surface. Photographed at 6.3x magnification	slide143.jpg 369.90 Kb Slide 143. Fragments were placed on the iris of the rabbit eye.
slide144.jpg 359.25 Kb Slide 144. 3 transplants are present on the eye. Photographed at 2x magnification	slide145.jpg 351.30 Kb Slide 145. The transplant at 10 o'clock is not angiogenic. The one at 2 o'clock has evoked a delicate capillary response on the 3rd day.	slide146.jpg 399.66 Kb Slide 146. This hyperplastic alveolar nodule from a mouse was highly angiogenic.	slide147.jpg 503.16 Kb Slide 147. This histologic slide of a "normal" angiogenic lobule shows the transplant sitting on top of the rabbit iris. Photographed at 6.3x magnification
slide148.jpg 570.48 Kb Slide 148. This histologic slide of a fragment of mild ductal hyperplasia is sitting on top of the rabbit iris. It was angiogenic. Photographed at 6.3x magnification ~ 30% of normal appearing lobules from cancer associated breasts were angiogenic in the rabbit iris assay but only 7% were angiogenic when removed from breast biopsies with no cancer. ~30% of ductal hyperplasia of the mild form were angiogenic.	slide149.jpg 287.91 Kb Slide 149. This diagram formulates the hypothesis that the angiogenic factor produced by the lobules stimulates fibroblasts to produce excess collagen as seen in fibrocystic change. ~35% of breasts with fibrocystic change have hyperplasias of ductal type.