26. Anthony T.W. Cheung, Lawrence J.T. Young, Peter C.Y. Chen, Chien Y. Chao, Assane Ndoye, Peter A. Barry, William J. Muller and Robert D. Cardiff. Microcirculation And Metastasis In A New Mouse Mammary Tumor Model System. Int. J. Oncology. 1997. 11:66-77. (ABSTRACT)(ILLUSTRATIONS)


A more complete image essay of this work can be found by clicking here.
LEGEND:
Figures 1A - 1F. Six (6) selected views of the pre- and post-transplantation tumor microcirculation of Met-1 videotaped under intravital microscopy (Magnification: 150x). 1A. A view of a pre-transplantation control showing the normal subcutaneous microcirculation at the neighboring site of the fat pad to which Met-1 was to be transplanted. This figure represents a view of the normal subcutaneous microcirculation, with the presence of three (3) parallel post-capillary venules (arrows), and with one (1) venule draining into a larger vein (marker). 1B. An internal view of a transplanted Met-1 tumor mass 4-days post-transplantation. The angiogenic vessels appear as black lines (neovessels) or fragmented black lines (sprouts to be joined to form a neovessel). The sprouts are indicated by markers. 1C. An internal view of a transplanted Met-1 tumor 10-days post-transplantation, showing a complex network of microvessels with arterio-venous anastomosis. 1D. A view of the internal microcirculation of Met-1 13-days post-transplantation, showing complex microvascular organization with tortuous vessels. 1E. A view of the external microcirculation on a Met-1 tumor 23-days post-transplantation, showing the straight external vessels. 1F. A view of the internal microcirculation inside the Met-1 tumor mass (in the same tumor in 1E) showing the complex and tortuous internal vessels.

LEGEND:
Figures 2A - 2B. Two (2) selected views of the internal microcirculation of Db-7 videotaped under intravital microscopy (Magnification: 150x). 2A. A view of the internal microcirculation in Db-7 13-days post-transplantation. Note the difference in organization pattern compared with Met-1 (see Figures 1D and 1F). 2B. A view of the internal microcirculation in Db-7 23-days post-transplantation. Note the relatively straight paths and the paucity of vasculature.

LEGEND:
Figures 3A-3B. Histological sections of Met-1 and Db-7 mammary tumors. 3A. H&E stained section showing a typical Met-1 transplanted mammary tumor. Note the papillary growth pattern and with small glands within the epithelium. The luminal space is filled with acellular, pink fluid, and apical blebs appear above he surface. Magnification is 400x. 3B. H&E section showing a typical Db-7 transplanted mammary tumor. Note the presence of pleomorphic cells with a solid growth pattern, the absence of glandular differentiation, and the large number of mitotic figures (11 in this field). Magnification is 400x.

LEGEND:
Figure 4. Comparison of the rate of metastasis (percent metastasis over time) from Met-1 and Db-7 mammary tumors. Each data point was calculated from the number of animals found with pulmonary metastasis divided by the total number of animals sacrificed for metastatic during a given time period multiplied by 100.

LEGEND:


LEGEND:

Other Example Images:

MT TUMORS
Db TUMORS
250 TUMORS

Back to Main Transgenic Page
Back to Top of Page

Last Updated: May 19, 1997